Circulation of Brucellaceae, Anaplasma and Ehrlichia spp. in borderline of Iran, Azerbaijan, and Armenia

Objective: To estimate the infection of ticks to Anaplasma, Ehrlichia, Babesia, Theileria, and Brucellaceae using molecular methods in borderline Iran, Azerbaijan, Armenia. Methods: Totally, 2 022 were collected from different livestock. Then, species diagnosed under stereomicroscope according valid morphological keys. Tick DNA was extracted followed by PCR detect Babesia ticks.Results: A total 498 males [24.62% (95% CI 22.76%-26.57%)], 741 females [36.64% 34.54%-38.79%)], 782 nymphs [38.67% 36.55%-40.84%)] 1 larva [0.04% 0.00%-0.28%)] identified. Among identified samples, we found four genera including Hyalomma, Rhipicephalus, Haemaphysalis, Dermacentor. Molecular assay revealed that prevalence Anaplasma or 22.02% 16.01%-29.06%) 15.03% 9.43%-22.26%), respectively. Phylogenetic analysis showed sp. had most similarity with centrale, platys, camelii, phagocytophilum, submitted GenBank. Furthermore, detected Ehrlichia bacterium ruminantium Mycoplana peli, However, no sign presence Theileria spp. seen studied samples.Conclusions: Anaplasmosis, ehrlichiosis brucellosis should be considered as important health threats northwestern Iran consistent monitoring on livestock performed regularly.